Figueiredo, J., Maia, M., Sousa Silva, M.*, Figueiredo, A.* (2017) Grapevine subtilase family: update on new sequences and nomenclature proposal. Frontiers in Plant Science 8: 716. doi: 10.3389/fpls.2017.00716

Vicente Miranda H, Szego ÉM, Oliveira LM, Breda C, Darendelioglu E, de Oliveira RM, Ferreira DG, Gomes MA, Rott R, Oliveira M, Munari F, Enguita FJ, Simões T, Rodrigues EF, Heinrich M, Martins IC, Zamolo I, Riess O, Cordeiro C, Ponces-Freire A, Lashuel HA, Santos NC, Lopes LV, Xiang W, Jovin TM, Penque D, Engelender S, Zweckstetter M, Klucken J, Giorgini F, Quintas A, Outeiro TF (2017) Glycation potentiates α-synuclein-associated neurodegeneration in synucleinopathies. Brain [Epub ahead of print, Apr 10]. doi: 10.1093/brain/awx056


Figueiredo, J., Costa, G., Maia, M., Paulo, O., Malhó, R., Sousa Silva, M.*, Figueiredo, A.* (2016) Revisiting Vitis vinífera subtilase gene family: a possible role in grapevine resistance against Plasmopara viticola. Frontiers in Plant Science 7: 1783. doi: 10.3389/fpls.2016.01783

Guerreiro, A., Figueiredo, J., Sousa Silva, M.*, Figueiredo, A.* (2016) Linking jasmonic acid to grapevine resistance against the biotrophic oomycete Plasmopara viticola. Frontiers in Plant Science – Special Research Topic on “Biotrophic plant-microbe interactions” 7, 565. doi: 10.3389/fpls.2016.00565

Maia, M., Monteiro, F., Sebastiana, M., Marques, A.P., Ferreira, A.E.N., Ponces Freire, A., Cordeiro, C., Figueiredo, A.*, Sousa Silva, M.* (2016) Metabolite extraction for high-throughput FTICR-MS-based metabolomics in grapevine. EuPA Open Proteomics 12: 4-9. doi: 10.1016/j.euprot.2016.03.002

Lebesgue N, da Costa G, Ribeiro RM, Ribeiro-Silva C, Martins GG, Matranga V, Scholten A, Cordeiro C, Heck AJ, Santos R. (2016) Deciphering the molecular mechanisms underlying sea urchin reversible adhesion: A quantitative proteomics approach. J Proteomics 138, 61-71. doi: 10.1016/j.jprot.2016.02.026

Lebesgue N., da Costa G., Ribeiro R.M., Ribeiro-Silva C., Martins G.G., Matranga V., Scholten A., Cordeiro C., Heck A.J., Santos R. (2016) Proteomic dataset of the sea urchin Paracentrotus lividus adhesive organs and secreted adhesive. Data Brief. 7: 1497-505. doi: 10.1016/j.dib.2016.04.002

Toubarro D., Gouveia A., Ribeiro R.M., Simões N., da Costa G., Cordeiro C., Santos R. (2016) Cloning, Characterization, and Expression Levels of the Nectin Gene from the Tube Feet of the Sea Urchin Paracentrotus lividus. Mar Biotechnol (NY). 18(3): 372-83. doi: 10.1007/s10126-016-9698-4

Vilas-Boas F, Bagulho A, Tenente R, Teixeira VH, Martins G, da Costa G, Jerónimo A, Cordeiro C, Machuqueiro M, Real C. (2016) Hydrogen peroxide regulates cell adhesion through the redox sensor RPSA. Free Radic Biol Med. 90, 145-57. doi: 10.1016/j.freeradbiomed.2015.11.019. (Epub 2015, Nov 18)

Marques A.P., Serralheiro M.L., Ferreira A.E., Freire A.P., Cordeiro C., Sousa Silva M. (2016) Metabolomics for undergraduates: Identification and pathway assignment of mitochondrial metabolites. Biochem Mol Biol Educ. 44(1), 38-54. doi: 10.1002/bmb.20919. (Epub 2015, Nov 5)


Oliveira BL, Morais M, Mendes F, Moreira IS, Cordeiro C, Fernandes PA, Ramos MJ, Alberto R, Santos I, Correia JD. (2015) Re(I) and Tc(I) complexes for targeting nitric oxide synthase: influence of the chelator in the affinity for the enzyme. Chem Biol Drug Des. 86(5), 1072-86. doi: 10.1111/cbdd.12575

Gaspar H, Bronze S, Ciríaco S, Queirós CR, Matias A, Rodrigues J, Oliveira C, Cordeiro C, Santos S. (2015) 4F-PBP (4′-fluoro-α-pyrrolidinobutyrophenone), a new substance of abuse: Structural characterization and purity NMR profiling. Forensic Sci Int. 252, 168-76. doi: 10.1016/j.forsciint.2015.05.003

Cal PM, Frade RF, Cordeiro C, Gois PM. (2015) Reversible lysine modification on proteins by using functionalized boronic acids. Chemistry 21(22),8182-7. doi: 10.1002/chem.201500127

da Costa G, Ribeiro-Silva C, Ribeiro R, Gilberto S, Gomes RA, Ferreira A, Mateus É, Barroso E, Coelho AV, Freire AP, Cordeiro C. (2015) Transthyretin Amyloidosis: Chaperone Concentration Changes and Increased Proteolysis in the Pathway to Disease. PLoS One 10(7), e0125392. doi: 10.1371/journal.pone.0125392


Cardoso LS, Suissas CE, Ramirez M, Antunes M, Pinto FR (2014) Comparison of alternative mixture model methods to analyze bacterial CGH experiments with multi-genome arrays. BMC Res Notes., 7, 148.

Simões RG, Diogo HP, Dias A, Oliveira MC, Cordeiro C, Bernardes CE, Minas Da Piedade ME. (2014) Thermal stability of simvastatin under different atmospheres. J Pharm Sci., 103, 241-248

Cal PM, Frade RF, Chudasama V, Cordeiro C, Caddick S, Gois PM. (2014) Targeting cancer cells with folic acid-iminoboronate fluorescent conjugates. Chem Commun (Camb) 50, 5261-5263

Teixeira, B., Fidalgo, L., Mendes, R., Costa, G., Cordeiro, C., Marques, A., Saraiva, J.A., Nunes, M.L. (2014) Effect of high pressure processing in the quality of sea bass (Dicentrarchus labrax) fillets: Pressurization rate, pressure level and holding time. Journal of Innovative Food Science and Emerging Technologies, 22, 31-39

Trübenbach K, da Costa G, Ribeiro-Silva C, Mesquita Ribeiro R, Cordeiro C, Rosa R (2014) Hypoxia-driven selective degradation of cellular proteins in jumbo squids during diel migration to the oxygen minimum zones. Marine Biology, 161, 575-584

Carrolo M, Pinto FR, Melo-Cristino J, Ramirez M. (2014) Pherotype influences biofilm growth and recombination in Streptococcus pneumoniae. PLoS One. 2014 Mar 19;9(3):e92138. doi: 10.1371/journal.pone.0092138.


Sousa Silva, M., Gomes, R.A., Ferreira, A.E.N., Ponces Freire, A., Cordeiro, C. (2013) The glyoxalase pathway: The first hundred years… and beyond. Biochemical Journal 453(1): 1-15 (Invited review). DOI: 10.1042/BJ20121743. link

Meireles, C., Costa, G., Guinote, I., Albuquerque, T., Botelho, A., Cordeiro, C., Freire, P. (2013) Pseudomonas putida are environmental reservoirs of antimicrobial resistance to β-lactamic antibiotics. World Journal Microbiology Biotechnology Feb 19. [Epub ahead of print]. DOI: 10.1007/s11274-013-1295-3. link

Teixeira, B.,Fidalgo, L., Mendes, R., Costa, G., Cordeiro, C., Marques, A., Saraiva, J.A.,  Nunes, M.L. (2013) Changes of Enzymes Activity and Protein Profiles Caused by High-Pressure Processing in Sea Bass (Dicentrarchus labrax) Fillets. Journal of Agricultural and Food Chemistry 61(11), 2851-2860. DOI: 10.1021/jf3049643link

Martins, A., Santos, M.S., Dias, C., Serra, P., Cachatra, V., Pais, J., Caio, J., Teixeira, V.H., Machuqueiro, M., Silva, M.S., Pelerito, A., Justino, J., Goulart, M., Silva, F.V., Rauter, A.P. (2013) Tuning bioactivity of new tensioactive deoxy glycosides with structure: antibacterial activity vs. selective cholinesterase inhibition rationalized by molecular docking. European Journal of Organic Chemistry 2013(8), 1448-1459. (Special issue dedicated to the centenary of the Portuguese Society of Chemistry – SPQ). DOI: 10.1002/ejoc.201201520. link

Rivaldi, J.D., Sousa Silva, M., Duarte, L.C., Ferreira, A.E.N., Cordeiro, C., Felipe, M.G.A., Ponces Freire, A., Mancilha, I.M. (2013) Metabolism of biodiesel-derived glycerol in probiotic Lactobacillus strains. Applied Microbiology and Biotechnology. 97(4), 1735-1743. DOI: 10.1007/s00253-012-4621-z. link


Lages, N.F., Cordeiro, C., Sousa Silva, M., Ponces Freire, A., Ferreira, A.E.N. (2012) Optimization of time-course experiments for kinetic model discrimination. PLoS One, 7(3), e32749, DOI: 10.1371/journal.pone.0032749, Full text.

Falé, P.L., Amaral, F., Madeira, P.J.A., Sousa Silva, M., Florêncio, M.H., Frazão, F.N., Serralheiro, M.L.M. (2012) Acetylcholinesterase inhibition, antioxidant activity and toxicity of Peumus boldus water extracts on HeLa and Caco-2 cell lines. Food and Chemical Toxicology 50(8), 2656-2662, DOI: 10.1016/j.fct.2012.04.049, link

Sousa Silva, M., Ferreira, A., Gomes, R., Tomás, A., Ponces Freire, A., Cordeiro, C. (2012) The glyoxalase pathway in protozoan parasites. International Journal of Medical Microbiology, 302 (4-5), 225-229 – Special issue “Molecular Principles of Parasitism” (Invited review). DOI: 10.1016/j.ijmm.2012.07.005, link

Gomes, R.A., Franco, C., Da Costa, G., Planchon, S., Renaut, J., Pinto, F., Sousa Silva, M., Coelho, A.V., Ponces Freire, A., Cordeiro, C. (2012) The proteome response to amyloid protein expression in vivo. PLoS One, 7(11): e50123. DOI:10.1371/journal.pone.0050123. Full text.

Da Silva, J.P., Kulasekharan, R., Cordeiro, C., Jockusch, S., Turro, N.J., Ramamurthy, V. (2012) Capsular Complexes of Nonpolar Guests with Octa Amine Host Detected in the Gas Phase. Organic Letters. 14(2), 560-563. DOI: 10.1021/ol203139v. link.

Guerreiro, A., da Costa, G., Gomes, R.A., Ribeiro-Silva, C., Gilberto, S., Mateus, E., Monteiro, E., Barroso, E., Coelho, A.V., Freire, A.P., Cordeiro, C. (2012) alpha-Synuclein aggregation in the saliva of familial transthyretin amyloidosis: a potential biomarker. Amyloid, 19(2), 74-80. link.

Cal, P.M., Vicente, J.B., Pires, E., Coelho, A.V., Veiros, L.F., Cordeiro, C., Gois, P.M. (2012) Iminoboronates: a new strategy for reversible protein modification. Journal of the American Chemical Society, 134 (24), 10299-305. DOI: 10.1021/ja303436y. link.

Friães, A., Pinto, F.R., Silva-Costa, C., Ramirez, M., Melo-Cristino, J. (2012) Group A streptococci clones associated with invasive infections and pharyngitis in Portugal present differences in emm types, superantigen gene content and antimicrobial resistance. BMC Microbiology, 12(1), 280, Full text.

Friães, A., Pinto, F.R., Silva-Costa, C., Ramirez, M., Melo-Cristino, J. (2012) Superantigen gene complement of Streptococcus pyogenes-relationship with other typing methods and short-term stability. European Journal of Clinical Microbiology and Infectious Diseases. E-pub August 2012. DOI: 10.1007/s10096-012-1726-3, link.

Valente, C., Hinds, J., Pinto, F., Brugger, S.D., Gould, K., Muhlemann, K., de Lencastre, H., Sa-Leao, R. (2012) Decrease in Pneumococcal Co-Colonization following Vaccination with the Seven-Valent Pneumococcal Conjugate Vaccine. PLoS One , 7(1), e30235. DOI: 10.1371/journal.pone.0030235. Full text.

Tendeiro, R., Foxall, R.B., Baptista, A.P., Pinto, F., Soares, R.S., Cavaleiro, R., Valadas, E., Gomes, P., Victorino, R.M.M., Sousa, A.E. (2012) PD-1 and its ligand PD-L1 are progressively up-regulated on CD4 and CD8 T-cells in HIV-2 infection irrespective of the presence of viremia. AIDS, 26(9), 1065-1071. DOI: 10.1097/QAD.0b013e32835374db. link.

Faria, D., Schlicker, A., Pesquita, C., Bastos, H., Ferreira, A.E., Albrecht, M., Falcão, A.O. (2012) Mining GO annotations for improving annotation consistency. PLoS One, 7(7), e40519. DOI: 10.1371/journal.pone.0040519. Full text.


Da Silva JP, Kulasekharan R, Cordeiro C, Jockusch S, Turro NJ, Ramamurthy V (2011) Capsular Complexes of Nonpolar Guests with Octa Amine Host Detected in the Gas Phase. Org Lett. 2011 Dec 23. [Epub ahead of print]

Severiano A, Pinto FR, Ramirez M, Carriço JA (2011) Adjusted Wallace coefficient as a measure of congruence between typing methods. J Clin Microbiol. 49(11), 3997-4000.

Severiano A, Carriço JA, Robinson DA, Ramirez M, Pinto FR (2011) Evaluation of jackknife and bootstrap for defining confidence intervals for pairwise agreement measures. PLoS One 6(5), e19539. DOI:10.1371/journal.pone.0019539 (Full Text)

da Costa G, Gomes RA, Guerreiro A, Mateus É, Monteiro E, Barroso E, Coelho AV, Freire AP, Cordeiro C (2011) Beyond genetic factors in familial amyloidotic polyneuropathy: protein glycation and the loss of fibrinogen’s chaperone activity. PLoS One 6(10), e24850. doi:10.1371/journal.pone.0024850 (Full Text).

Ribeiro-Silva C, Gilberto S, Gomes RA, Mateus É, Monteiro E, Barroso E, Coelho AV, da Costa G, Freire AP, Cordeiro C (2011) The relative amounts of plasma transthyretin forms in familial transthyretin amyloidosis: a quantitative analysis by Fourier transform ion-cyclotron resonance mass spectrometry. Amyloid 18(4), 191-9.

Barata L, Sousa Silva M, Schuldt L, Ferreira AEN, Gomes RA, Tomás AM, Weiss MS, Ponces Freire A, Cordeiro C (2011) Enlightening the molecular basis of trypanothione specificity in trypanosomatids: mutagenesis of Leishmania infantum glyoxalase II. Exp Parasitol 129(4), 402-8.

Fortes AM, Agudelo-Romero P, Silva MS, Ali K, Sousa L, Maltese F, Choi YH, Grimplet J, Martinez-Zapater JM, Verpoorte R, Pais MS (2011) Transcript and metabolite analysis in Trincadeira cultivar reveals novel information regarding the dynamics of grape ripening. BMC Plant Biology 11, 149. doi:10.1186/1471-2229-11-149.

Sá-Leão R, Pinto FR, Aguiar S, Nunes S, Carriço JA, Frazão N, Gonçalves-Sousa N, Melo-Cristino J, de Lencastre H, and Ramirez M (2011) Analysis of invasiveness of pneumococcal serotypes and clones circulating in Portugal before widespread use of conjugate vaccines revealing heterogeneous behavior of clones expressing the same serotype, J Clin Microbiol., 49(4),1369-75.


Barata, L., Sousa Silva, M., Schuldt, L., Tomás, A.M., Ferreira, A.E.N., Weiss, M.S., Ponces Freire, A., Cordeiro, C. (2010) Cloning, Expression, Purification, Crystallization and Preliminary X-ray Diffraction Analysis of Glyoxalase I from Leishmania infantum, Acta Crystallographica Section F 66, 571-4.

da Costa G,. Correia CF, Gomes RJ, Freire A, Monteiro E, Barroso E, Coelho AV , Outeiro TF, Freire AP and Cordeiro C. (2010) A non-invasive method based on saliva to characterize TTR in PAF patients using FT-ICR high-resolution mass spectrometry, Proteomics Clin. Appl. 4, 674-8.

Lamy E, da Costa G, Santos R, Capela E Silva F, Potes J, Pereira A, Coelho AV, Sales Baptista E. (2010) Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome, J Anim Physiol Anim Nutr (Berl), doi: 10.1111/j.1439-0396.2010.01055.x.

Lamy E, Graca G, da Costa G, Franco C, Capela E Silva F, Sales Baptista E, Coelho AV. (2010) Changes in mice whole saliva soluble proteome induced by tannin-enriched diets, Proteome Sci. 15;8(1), 65.

Oliveira BL, Raposinho PD, Mendes F, Santos IC, Santos IS, Ferreira A, Cordeiro C, Freire AP, Correia JDC (2010) Targeting nitric oxide synthase with 99mTc/Re tricarbonyl complexes containing pendant guanidino or isothiourea moieties, J Organometal. Chem., doi:10.1016/j.jorganchem.2010.09.019.

Oliveira BL, Raposinho PD, Mendes F, Figueira A, Santos I, Ferreira A, Cordeiro C, Freire AP, Correia JD (2010) Re and Tc Tricarbonyl Complexes: From the Suppression of NO Biosynthesis in Macrophages to in Vivo Targeting of Inducible Nitric Oxide Synthase, Bioconjug Chem, 21(12), 2168-72.

Carrolo M, Frias MJ, Pinto FR, Melo-Cristino J, Ramirez M. (2010) Prophage Spontaneous Activation Promotes DNA Release Enhancing Biofilm Formation in Streptococcus pneumoniae, PLoS One 5(12), e15678.

Aguiar SI, Pinto FR, Nunes S, Serrano I, Melo-Cristino J, Sá-Leão R, Ramirez M, de Lencastre H (2010) Increase of Denmark14-230 clone as a cause of pneumococcal infection in Portugal within a background of diverse serotype 19A lineages, J Clin Microbiol. 48(1), 101-8.


R. Santos, G. da Costa, C. Franco, P. Alves, P. Flammang and A. V. Coelho (2009) First insights into the biochemistry of tube foot adhesives from the sea urchin Paracentrotus lividus, Marine Biotechnol, 11, 686-98.

Toubarro, D., M. Lucena-Robles, G. Nascimento, da Costa G., R. Montiel, A. Coelho, N. Simões (2009) An apoptosis induncing serine protease secreted by the entomopathogenic nematode Steinernema caopocapsa, Int J Parasitol., 39, 1319-30.

Chaves I, Pinheiro C, Paiva JAP, Planchon S, Sergeant K, Renaut J, JGraça JA, da Costa G, Coelho AV, Ricardo CPP (2009) Proteomic evaluation of wound-healing processes in Potato (Solanum tuberosum L.) tuber tissue. Proteomics, 9, 4154-75.

Campos A, da Costa G, Coelho AV and Fevereiro P (2009) Identification of bacterial protein markers and enolase as a plant response protein to infection of Olea europaea L. subsp. europaea by Pseudomonas savastanoi pv savastanoi. Eur J Plant Pathol, 125, 603-16.

Lamy, E., G. Da Costa, R. Santos, F. Capela e Silva, J. Potes, A. Pereira, A.V. Coelho A and E. Sales Baptista (2009) Sheep and goat saliva proteome analysis: a useful tool for ingestive behavior research?, Physiol Behav, 98, 393-40.

Pinto F.R.J (2009) A Probabilistic Approach to Study Yeast’s Gene Regulatory Network. Comput Sci Syst Biol, 2, 044-050.

Carrolo M, Pinto FR, Melo-Cristino J, Ramirez M, (2009) Pherotypes are driving genetic differentiation within Streptococcus pneumonia. BMC Microbiol., 9, 191.

da Costa G, Gomes R, Correia CF, Freire A, Monteiro E, Martins A, Barroso E, Coelho AV, Outeiro TF, Ponces Freire A, Cordeiro C.(2009) Identification and quantitative analysis of human transthyretin variants in human serum by Fourier transform ion-cyclotron resonance mass spectrometry. Amyloid. 16, 201-207.

Faria D, Ferreira AE, Falcão AO (2009) Enzyme classification with peptide programs: a comparative study. BMC Bioinformatics, 24, 231

Oliveira B.L., Correia J.D., Raposinho P.D., Santos I., Ferreira A., Cordeiro C., Freire A.P. (2009) Re and 99mTc organometallic complexes containing pendant l-arginine derivatives as potential probes of inducible nitric oxide synthase. Dalton Transactions. Jan 7, 1: 152-162. abstract


Pinto F.R., Melo-Cristino J., Ramirez M. (2008) A Confidence Interval for the Wallace Coefficient of Concordance and Its Application to Microbial Typing Methods. PLoS ONE, 3(11):e3696. doi:10.1371/journal.pone.0003696 Full text

Fortes A.M., Santos F., Choi Y.H., Silva M.S., Figueiredo A., Sousa L., Pessoa F., Santos B.A., Sebastiana M., Palme K., Malhó R., Verpoorte R., Pais M.S. (2008) Organogenic nodule development in hop (Humulus lupulus L.): transcript and metabolic responses.
BMC Genomics. 9: 445. Full text

Gomes R.A, Oliveira L.M., Silva M., Ascenso C, Quintas A., Costa G., Coelho A.V., Sousa Silva M., Ferreira A.E., Ponces Freire A., Cordeiro C.(2008) Protein glycation in vivo: functional and structural effects on yeast enolase. Biochem J, 416: 317-326. abstract

Sousa Silva, M., Barata, L., Ferreira, A.E.N., Romão, S., Tomás, A.M., Ponces Freire, A., Cordeiro, C. (2008) Catalysis and Structural Properties of Leishmania infantum Glyoxalase II: Trypanothione Specificity and Phylogeny, Biochemistry, 47: 195-204. DOI: 10.1021/bi700989m. abstract

Vicente Miranda, H. , Ferreira, A.E.N., Quintas, A., Cordeiro, C., Ponces Freire, A. (2008)
Measuring Intracellular Enzyme Concentrations. Biochemical and Molecular Biology Education, 36:135-138. Full text

Ispolnov K., Gomes R.A., Silva M.S., Freire A.P. (2008) Extracellular methylglyoxal toxicity in Saccharomyces cerevisiae: role of glucose and phosphate ions. Journal of Applied Microbiology, 104: 1092-1102. abstract

Gomes, R., Vicente Miranda, H., Sousa Silva, M., Graça, G., Coelho, A. V., Ferreira, A.E.N., Cordeiro, C., Ponces Freire, A. (2008) Protein glycation and methylglyoxal metabolism in yeast: finding peptide needles in protein haystacks. FEMS Yeast Research, 8: 174-181. abstract


Neng, N.R., Cordeiro,C., Ponces Freire, A., Nogueira, J.M.F. (2007) Determination of glyoxal and methylglyoxal in environmental and biological matrices by stir bar sorptive extraction with in situ derivatization. Journal of Cromatography A, 1169: 47-52. abstract

Farinha, C.M., Ponces Freire, A. (2007) Teaching biochemistry at Lisbon University – Facing the challenge of the Bologna Declaration in the 25th anniversary of the biochemistry course. Biochemical and Molecular Biology Education, 35: 422-424. Full text

Oliveira, L.M.A., Cordeiro, C., Ponces Freire, A., Ascenso, C., Quintas, A. (2007).
Unveiling heme proteins conformational stability through a UV absorbance ratio method.
Analytical Biochemistry, 371: 253-255. abstract

Maia, L., Duarte, R., Moura, J., Ponces Freire, A., Mira, L. (2007) NADH oxidase activity of rat and human liver xanthine oxidoreductase: potential role in superoxide production.
Journal of Biological Inorganic Chemistry, 12: 777-787. abstract


Gomes, R., Vicente Miranda, H., Sousa Silva, M., Graça, G., Coelho, A. V., Ferreira, A.E.N., Cordeiro, C., Ponces Freire, A. (2006) Yeast protein glycation in vivo by methylglyoxal Molecular modification of glycolytic enzymes and heat shock proteins.
FEBS Journal, 273: 5273-5287. abstract

Trincao J., Sousa Silva M., Barata L., Bonifacio C., Carvalho S., Tomas A.M., Ferreira, A.E.N., Cordeiro C., Ponces Freire A., Romao M.J. (2006) Purification, crystallization and preliminary X-ray diffraction analysis of the glyoxalase II from Leishmania infantum.
Acta Crystallograph Sect F Struct Biol Cryst Commun. 62: 805-807. abstract


Vicente Miranda, H. , Ferreira, A.E.N., Cordeiro, C., Ponces Freire, A. (2005) Kinetic assay for measurement of enzyme concentration in situ. Analytical Biochemistry, 354:148-150. abstract

Gomes, R., Sousa Silva, M., Vicente Miranda, H. , Ferreira, A.E.N., Cordeiro, C., Ponces Freire, A. (2005) Protein glycation in Saccharomyces cerevisiae : Argpyrimidine formation and methylglyoxal catabolism. FEBS Journal , 272(17): 4521-4531. abstract

Abecasis, J.P.R., Ferreira, A.E.N., Vicente Miranda, H. , Gomes, R., Sousa Silva, M., Cordeiro, C., Ponces Freire, A. (2005) Parameter estimation in multi-enzyme systems using evolutionary algorithms. BKDB2005 – Bioinformatics: Knowledge Discovery in Biology . Faculdade de Ciências de Lisboa Ed. pp 48-52. Full text

Sousa Silva, M. , Ferreira, A.E.N., Tomás, A.M., Cordeiro, C., Ponces Freire, A. (2005)  Quantitative assessment of the glyoxalase pathway in Leishmania infantum as a therapeutic target by modelling and computer simulation. FEBS Journal 272(10): 2388-2398. abstract

do Rosario PM , Cordeiro CA , Freire AP , Nogueira JM (2005) Analysis of methylglyoxal in water and biological matrices by capillary zone electrophoresis with diode array detection.
Electrophoresis, 26(9):1760-1767. abstract

Gomes, R., Sousa Silva, M. , Quintas, A., Cordeiro, C., Freire, A., Pereira, P., Martins, A., Monteiro, E., Barroso, E., Ponces Freire, A. (2005) Argpyrimidine, a methylglyoxal-derived advanced glycation end-product in familial amyloidotic polyneuropathy. Biochemical Journal 385: 339-345. abstract


Ponces-Freire, A., Ferreira, A.E., Gomes, R. and Cordeiro, C. (2003) Antiglycation defences in yeast. Biochem. Soc. Trans. 31 : 1409-1412. abstract

Ferreira, A.E., Ponces-Freire, A. and Voit, E.O. (2003) A quantitative model of the generation of N-e-carboxymethyl-lysine in the Maillard reaction between collagen and glucose. Biochem. J. 376 : 109-121. abstract


Ahmed, N., Argirov, O., Minhas, H., Cordeiro, C. and Thornaley, P.J. (2002) Assay of advanced glycation endproducts (AGEs) by chromatographic assay with derivatisation by aminoquinolyl-N-hydroxysuccinimidyl-carbamate and application to N-e-carboxymethyl-lysine- and N-e-(1-carboxyethyl)lysine-modified albumin, Biochem. J. 364 : 1-14. abstract

Older than 2002

Martins, A., Mendes, P., Cordeiro, C. and Ponces Freire, A. (2001) In situ kinetic analysis of glyoxalase I and glyoxalase II in Saccharomyces cerevisiae, Eur. J. Biochem. 268 : 3930-3936. abstract

Martins, A., Cordeiro, C. and Ponces Freire, A. (2001) In situ analysis of methylglyoxal metabolism in Saccharomyces cerevisiae , FEBS Letters, 499 : 41-44. abstract

Martins, A., Cordeiro, C. and Ponces Freire, A. (1999) Glyoxalase II in Saccharomyces cerevisiae: In situ kinetics using the 5,5′-dithiobis(2-nitrobenzoic) acid assay, Arch. Biochem. Biophys. 366 : 15-20. abstract

Voit, E.O. and Ferreira, A. (1998) Buffering in Models of Integrated Biochemical Systems, J. Theor. Biol. , 268 : 3930-3936.

Cordeiro, C. and Ponces Freire, A. (1996) Methylglyoxal assay in cells as 2-methylquinoxaline using 1,2-diaminobenzene as derivatizing reagent, Anal. Biochem. 234 : 221-224. abstract

Cordeiro, C. and Ponces Freire, A. (1996) Methylglyoxal, glyoxalase I and oxidative stress, Biochem. Soc. Transactions. 24 : 472S. abstract

Cordeiro, C. and Ponces Freire, A. (1995) Digitonin permeabilization of Saccharomyces cerevisiae cells for in situ enzyme assay, Anal. Biochem. 229 . 145-148. abstract

Cordeiro, C. and Ponces Freire, A. (1994) Protein Determination in permeabilized yeast cells, Anal. Biochem. 223 . 321-323. abstract